diff --git a/core/relinker/CMakeLists.txt b/core/relinker/CMakeLists.txt
index e4441e4f19..df54dba194 100644
--- a/core/relinker/CMakeLists.txt
+++ b/core/relinker/CMakeLists.txt
@@ -523,6 +523,9 @@ if(BUILD_TESTING)
         set_tests_properties(needed_libraries PROPERTIES TIMEOUT 30)
         add_test(NAME macos_output COMMAND ${Python3_EXECUTABLE}
             ${CMAKE_CURRENT_SOURCE_DIR}/relinker/tests/test_macos_output.py $<TARGET_FILE:relinker>)
+        add_test(NAME macos_tls_overlap COMMAND ${Python3_EXECUTABLE}
+            ${CMAKE_CURRENT_SOURCE_DIR}/relinker/tests/test_macos_tls_overlap.py $<TARGET_FILE:relinker>)
+        set_tests_properties(macos_tls_overlap PROPERTIES TIMEOUT 60)
         add_test(NAME guest_host_libc COMMAND ${Python3_EXECUTABLE}
             ${CMAKE_CURRENT_SOURCE_DIR}/relinker/tests/test_guest_host_libc.py $<TARGET_FILE:relinker>)
         add_library(guest_module_args_host SHARED elfpatcher/tests/GuestModuleArgsHost.cpp)
diff --git a/core/relinker/elfpatcher/src/macos/MacOsImage.cpp b/core/relinker/elfpatcher/src/macos/MacOsImage.cpp
index 838d0d6c8b..196084a66e 100644
--- a/core/relinker/elfpatcher/src/macos/MacOsImage.cpp
+++ b/core/relinker/elfpatcher/src/macos/MacOsImage.cpp
@@ -218,16 +218,19 @@ TlsScan ScanTls(const std::vector<std::uint8_t>& source, const std::vector<Domai
             scan.Tls = &header;
         }
         if (header.Type != 1 || (header.Flags & 1) == 0) continue;
+        std::uint64_t decodedEnd = 0;
         for (auto instruction = instructions.lower_bound(header.MappedAddress); instruction != instructions.end() && *instruction - header.MappedAddress < header.FileSize; ++instruction) {
             const auto offset = *instruction - header.MappedAddress;
             const auto* bytes = source.data() + header.Offset + offset;
             const auto info = decoder.DecodeInstruction(bytes, header.FileSize - offset);
             const auto rva = image.GetRva(header.MappedAddress + offset, info.Length);
+            const bool insidePrevious = *instruction < decodedEnd;
+            decodedEnd = std::max(decodedEnd, *instruction + info.Length);
             if (info.HasBranchTarget && !info.HasRipRelativeDisp) {
                 const auto target = static_cast<std::int64_t>(rva) + static_cast<std::int64_t>(info.Length) + info.BranchDisp;
                 if (target >= 0 && target <= std::numeric_limits<std::uint32_t>::max()) scan.BranchTargets.insert(static_cast<std::uint32_t>(target));
             }
-            if (info.SegmentPrefix == 0) continue;
+            if (info.SegmentPrefix == 0 || (insidePrevious && info.SegmentPrefix != 0x64)) continue;
             const auto position = info.OpcodeOffset;
             bool hasOperandSizePrefix = false;
             bool supportedPrefixes = info.SegmentPrefix == 0x64;
diff --git a/core/relinker/relinker/tests/test_macos_tls_overlap.py b/core/relinker/relinker/tests/test_macos_tls_overlap.py
new file mode 100644
index 0000000000..0eaeb6396d
--- /dev/null
+++ b/core/relinker/relinker/tests/test_macos_tls_overlap.py
@@ -0,0 +1,50 @@
+"""Check the macOS TLS scan against decodings that start inside another instruction."""
+from pathlib import Path
+import subprocess
+import sys
+import tempfile
+
+from test_macos_output import MachO
+from test_tls_function_coverage import TLS_LOAD, add_alias, make_image
+
+
+def convert(relinker, directory, name, image):
+    source = Path(directory) / f"{name}.elf"
+    output = Path(directory) / name
+    source.write_bytes(bytes(image))
+    result = subprocess.run([str(relinker), "--macos", "--skip-sce-module", str(source), str(output)],
+                            capture_output=True, text=True, timeout=60)
+    return result, output
+
+
+def check_split_function(relinker, directory):
+    immediate = bytes.fromhex("48 b9 00 00 00 65 2e 62 69 6e")
+    body = TLS_LOAD + bytes.fromhex("eb 00 8b 40 f0") + immediate + b"\xc3" * 8
+    image = add_alias(make_image("register", "symbol", 0x55, body), 0x0c, 0x1256)
+    result, output = convert(relinker, directory, "function-begins-inside-instruction", image)
+    if result.returncode != 0:
+        raise SystemExit(f"function-begins-inside-instruction: relinker failed: {result.stdout}{result.stderr}")
+    data = output.read_bytes()
+    code = b"".join(data[lo:hi] for lo, hi in MachO(data).executable_ranges())
+    if immediate not in code:
+        raise SystemExit("function-begins-inside-instruction: the instruction around the GS-prefixed decoding changed")
+
+
+def check_gs_access_refused(relinker, directory):
+    image = make_image("register", "unwind", body=bytes.fromhex("65 48 8b 04 25 28 00 00 00") + b"\xc3")
+    result, output = convert(relinker, directory, "gs-access", image)
+    message = result.stdout + result.stderr
+    if result.returncode != 2 or "Unsupported macOS guest TLS instruction" not in message or output.exists():
+        raise SystemExit(f"gs-access: a GS access at an instruction start was not refused: {message}")
+
+
+def main():
+    relinker = Path(sys.argv[1]).resolve()
+    with tempfile.TemporaryDirectory(prefix="anyps5-macos-tls-overlap-") as directory:
+        check_split_function(relinker, directory)
+        check_gs_access_refused(relinker, directory)
+    print("macOS TLS overlap checks passed")
+
+
+if __name__ == "__main__":
+    main()